A STUDY OF OXIDATIVE PHOSPHORYLATION WITH O18-LABELED INORGANIC PHOSPHATE
نویسندگان
چکیده
منابع مشابه
A study with O18 of adenosine triphosphate formation in oxidative phosphorylation.
In a previous communication from this laboratory (1) a reaction of inorganic phosphate with water was described which occurred during phosphorylation coupled to oxidation in the electron transport system in liver mitochondria. This reaction was followed by observing the replacement of 018 in labeled inorganic phosphate by normal oxygen from water concomitant with the occurrence of phosphorylati...
متن کاملPhosphorylation by inorganic phosphate of sarcoplasmic membranes.
The calcium transport protein of the sarcoplasmic reticulum accepts inorganic phosphate rapidly when phosphorylation is initiated either by the addition of phosphate or magnesium ions to the calcium free protein. Phosphorylation proceeds much more slowly when it is initiated by the addition of the calcium chelatro ethyleneglycol-bis (beta-aminoethyl ether)-N,N'-tetraacetic acid (EGTA) to the ph...
متن کاملThe apparent absolute requirement of adenosine diphosphate for the inorganic phosphate--water exchange of oxidative phosphorylation.
With suitable submitochondrial particles from the heart, the rate of the inorganic phosphate e HOH exchange during substrate oxidation in the absence of ADP is less than l/1000 of the rate in the presence of ADP. The concentration dependence of the exchange on ADP shows simple saturation behavior with an apparent K, of 0.32 mM. For net oxidative phosphorylation under similar conditions, but wit...
متن کاملO18 and P32 exchange reactions of mitochondria in relation to oxidative phosphorylation.
The study of oxidative phosphorylation’ by the classical techniques of fractionation and isolation of the purified reactants has not been feasible because of the lability of the reaction systems. Consequently indirect approaches to the problem have been adopted. This paper reports results from one such approach; namely, study by the use of Pa2 and 01* of enzymic exchange reactions of phosphate ...
متن کاملMicropuncture Study of Inorganic Phosphate Excretion
The mechanism by which the mammalian kidney excretes inorganic phosphate has not been fully elucidated. The only micropuncture data available are those of Walker and Hudson (1), and they deal with the mode of phosphate excretion in two amphibian species. These investigators reported that the inorganic phosphate concentration rises progressively along the proximal and distal nephron of the frog ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1953
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)66231-3